Application
staining PDGF Receptor alpha in human ovarian carcinoma by Immunohistochemistry (FFPE-sections).Application
Human Ovarian Carcinoma stained with anti-PDGFR alpha antibody| Product Name | PDGF Receptor alpha antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDGF Receptor alpha predilutedPlatelet derived growth factor receptor (PDGFR) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF AA, BB or AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras, Raf and ERK 1 and 2 pathway. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) corresponding to C terminal of human PDGFR alpha. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC-P: Use at an assay dependent dilution. Staining of formalin fixed tissues requires boiling tissue sections in 10mMcitrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD140A MGC74795 PDGFR2 RHEPDGFRA |
|---|---|
| Target | PDGFR alpha |
| Cellular Localization | Cell Membrane and Cytoplasmic. |
Application
staining PDGF Receptor alpha in human ovarian carcinoma by Immunohistochemistry (FFPE-sections).
Application
Human Ovarian Carcinoma stained with anti-PDGFR alpha antibody| Positive Control | Human skin and Ovarian Carcinoma |
|---|---|
| Application Notes | IHC-P: Use at an assay dependent dilution. Staining of formalin fixed tissues requires boiling tissue sections in 10mMcitrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide |
