| Product Name | PDGF Receptor alpha (phospho Tyr754) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDGF Receptor alpha ( phospho Y754 )Platelet-derived growth factor receptor (PDGFR) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo- or heterodimerization upon ligand binding. The phosphorylation of tyrosine residues in the activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras, Raf and ERK 1 and 2 pathway. Phosphorylation of tyrosine 754 is thought to initiate specific signal-transduction pathways distinct from those initiated via homodimeric receptor complexes, as tyrosine 754 has not been shown to be phosphorylated in those complexes. |
| Immunogen | Synthetic phospho peptide (Human) containing Tyrosine 754. |
| Modification | p-Tyr754 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: Use at a concentration of 0.35 - 1 μg/ml. Detects a band of approximately 170-185 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatXenopus |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD140A MGC74795 PDGFR2 RHEPDGFRA PDGFR alpha (phospho Y754) PDGFR alpha (phospho Tyr754) PDGFR alpha (pY754) PDGFR alpha (pTyr754) PDGFR alpha phospho Y754 PDGFR alpha phospho Tyr754 Phospho PDGFR alpha (Y754) Phospho PDGFR alpha (Tyr754) Phosp |
|---|---|
| Target | PDGFR alpha |
| Tissue Specificity | PDGFR beta (50%) has not been tested, but is not expected to react. |
| Cellular Localization | Cell Membrane and Cytoplasmic. |
| Positive Control | NIH3T3 cells +/- PDGF |
|---|---|
| Application Notes | WB: Use at a concentration of 0.35 - 1 μg/ml. Detects a band of approximately 170-185 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 |
