Application
'Western Blot: HIF Prolyl Hydroxylase 3 Antibody - Western Blot analysis of human PHD3, using . Samples: MEF whole cell lysate.'| Product Name | PHD3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PHD3HIF prolyl hydroxylase 3 is a prolyl hydroxylase that modifies HIF alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HIF prolyl hydroxylase 3, specifically, catalyzes the posttranslational formation of 4 hydroxyproline in HIF alpha proteins. It hydroxylates HIF 1 alpha at Pro(564) and HIF 2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may also play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissues, promoting cell death through a caspase-dependent mechanism. |
| Immunogen | A synthetic peptide made to the C-terminus of the human protein sequence of HIF prolyl hydroxylase 3. [LocusLink ID 112399] |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000 - 1/3000 (ECL). Detects a band of approximately 27 kDa (predicted molecular weight: 28.6 kDa) in HeLa and MEF cell lysates. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ21620 HIFPH3 MGC125998 MGC125999 PHD3 |
|---|---|
| Target | PHD3 |
| Cellular Localization | Cell Membrane and Nuclear |
Application
'Western Blot: HIF Prolyl Hydroxylase 3 Antibody - Western Blot analysis of human PHD3, using . Samples: MEF whole cell lysate.'| Application Notes | WB: Use at a dilution of 1/1000 - 1/3000 (ECL). Detects a band of approximately 27 kDa (predicted molecular weight: 28.6 kDa) in HeLa and MEF cell lysates. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4 degrees C. |
| Storage Buffer | Tris-citrate/phosphate [pH 7-8] containing 0.01% sodium azide |
