WB
Western blot analysis of PHD3 on different lysates with Rabbit anti-PHD3 antibody at 1/1,000 dilution. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | PHD3 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant full length protein within human PHD3. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EGLN3 |
|---|---|
| Gene Synonyms | PHD3 HIFPH3 HIFP4H3 |
| Gene Full Name | egl-9 family hypoxia inducible factor 3 |
| Gene Summary | Enables peptidyl-proline 4-dioxygenase activity. Involved in protein hydroxylation. Located in cytosol and nucleus. Implicated in renal cell carcinoma. Biomarker of clear cell renal cell carcinoma. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Egl 9 family hypoxia inducible factor 3 antibody Egl nine homolog 3 (C. elegans) antibody Egl nine homolog 3 antibody Egl nine like protein 3 isoform antibody EGL9 homolog of C. elegans 3 antibody EGLN3 antibody EGLN3_HUMAN antibody Factor responsive smooth muscle protein antibody HIF Prolyl Hydroxylase 3 antibody HIF-PH3 antibody Egl 9 family hypoxia inducible factor 3 antibody Egl nine homolog 3 (C. elegans) antibody Egl nine homolog 3 antibody Egl nine like protein 3 isoform antibody EGL9 homolog of C. elegans 3 antibody EGLN3 antibody EGLN3_HUMAN antibody Factor responsive smooth muscle protein antibody HIF Prolyl Hydroxylase 3 antibody HIF-PH3 antibody HIF-prolyl hydroxylase 3 antibody HIFP4H3 antibody HIFPH3 antibody HPH-1 antibody HPH-3 antibody Hypoxia-inducible factor prolyl hydroxylase 3 antibody P4H3 antibody PHD3 antibody Prolyl Hydroxylase Domain Containing Protein 3 antibody Prolyl hydroxylase domain-containing protein 3 antibody SM20 antibody |
| Molecular Weight(MW) | 27 kDa(Observed band size: 30 kDa) |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Western blot analysis of PHD3 on different lysates with Rabbit anti-PHD3 antibody at 1/1,000 dilution. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
