Western blot
0.2-1 ug/mlPositive Control: Human brainWestern blot
Host: Rabbit Target Name: EGLN3 Sample Tissue: Human Adult Placenta Antibody Dilution: 1.0ug/ml| Product Name | Rabbit anti-EGLN3 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ21620, HIFPH3, MGC125998, MGC125999, PHD3 |
| Immunogen | The immunogen for anti-EGLN3 antibody: synthetic peptide directed towards the C terminal of human EGLN3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EGLN3 |
|---|---|
| Gene Synonyms | FLJ21620 HIFPH3 MGC125998 MGC125999 PHD3 |
| Gene Full Name | Egl nine homolog 3 (C. elegans) |
| Gene Summary | EGLN3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. EGLN3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. EGLN3 promotes cell death through a caspase-dependent mechanism. |
| Alternative Names | FLJ21620 HIFPH3 MGC125998 MGC125999 PHD3 |
| Molecular Weight(MW) | 27kDa |
| Sequence | 239 amino acids |
Western blot
0.2-1 ug/mlPositive Control: Human brain
Western blot
Host: Rabbit Target Name: EGLN3 Sample Tissue: Human Adult Placenta Antibody Dilution: 1.0ug/ml| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
