Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).Application
EGLN3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | EGLN3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Egl nine homolog 3, HPH-1, Hypoxia-inducible factor prolyl hydroxylase 3, HIF-PH3, HIF-prolyl hydroxylase 3, HPH-3, Prolyl hydroxylase domain-containing protein 3, PHD3, EGLN3 |
| Product description | EGLN3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism (By similarity).1) Sato, M., et al. Exp. Cell Res. 316(17):2871-2882(2010) |
| Immunogen | This EGLN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-231 amino acids from the C-terminal region of human EGLN3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EGLN3 |
|---|---|
| Alternative Names | Egl nine homolog 3 HPH-1 Hypoxia-inducible factor prolyl hydroxylase 3 HIF-PH3 HIF-prolyl hydroxylase 3 HPH-3 Prolyl hydroxylase domain-containing protein 3 PHD3 EGLN3 |
| Molecular Weight(MW) | 27 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).
Application
EGLN3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
