Application
Immunohistochemical staining of PMCA4 ATPase and CaSRO in human corneal epithelium (hCE). (A) PMCA4 ATPase staining (green) on cell membranes of all cells in each layer of hCE. (B) CaSRO staining (red) on cell membranes of hCE. (C) Merged image.| Product Name | PMCA4 ATPase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [JA9] to PMCA4 ATPaseThe protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Immunogen | Purified human erythrocyte PMCA. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | JA9 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-FIHC-PIPWB |
| ICC/IF: 1:300: |
|
| Species Reactivity | HumanMousePigRatAmphibiansRabbitBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | PMCA4b ATP2B2 PMCA4x ATP2B4 MXRA1 PMCA4 ATPase Ca++ transporting plasma membrane 4 |
|---|---|
| Target | PMCA4 ATPase |
| Tissue Specificity | The epitope sequence of this antibody is common to both PMCA4a and 4b ATPase. |
Application
Immunohistochemical staining of PMCA4 ATPase and CaSRO in human corneal epithelium (hCE). (A) PMCA4 ATPase staining (green) on cell membranes of all cells in each layer of hCE. (B) CaSRO staining (red) on cell membranes of hCE. (C) Merged image.| Application Notes | ICC/IF: 1:300: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | PBS containing 0.05 % sodium azide and 1 mg/ml BSA |
