Application
ICC/IF analysis of C2C12 cells using Polycystin 2 antibody (red) and DAPI (blue). (C) Merged images of panels A and B.Application
IHC-P analysis of rat kidney using Polycystin 2 antibody at a dilution of 1:100 followed by (A) Alexa Fluor 594 secondary antibody and (B) DAB staining. PT: proximal tubule. DT: distal tubule.Application
WB analysis of rat kidney membrane proteins (lanes 1 and 3) and mouse kidney lysates (lanes 2 and 4) using Polycystin 2 antibody at a dilution of 1:200 in the absence (lens 1 and 2) or presence (lens 3 and 4) of blocking peptide.| Product Name | Polycystin 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Polycystin 2This gene encodes a member of the polycystin protein family. The encoded protein is a multi-pass membrane protein that functions as a calcium permeable cation channel, and is involved in calcium transport and calcium signaling in renal epithelial cells. This protein interacts with polycystin 1, and they may be partners in a common signaling cascade involved in tubular morphogenesis. Mutations in this gene are associated with autosomal dominant polycystic kidney disease type 2. [provided by RefSeq, Mar 2011] |
| Immunogen | Peptide (C)ERWESDDAASQISH, corresponding to amino acid residues 914-927 of human TRPP1 (Accession Q13563). Intracellular, C-terminus. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PKD4 PKD2 TRPP2 APKD2 polycystic kidney disease 2 (autosomal dominant) PC2 Pc-2 |
|---|---|
| Target | Polycystin 2 |
Application
ICC/IF analysis of C2C12 cells using Polycystin 2 antibody (red) and DAPI (blue). (C) Merged images of panels A and B.
Application
IHC-P analysis of rat kidney using Polycystin 2 antibody at a dilution of 1:100 followed by (A) Alexa Fluor 594 secondary antibody and (B) DAB staining. PT: proximal tubule. DT: distal tubule.
Application
WB analysis of rat kidney membrane proteins (lanes 1 and 3) and mouse kidney lysates (lanes 2 and 4) using Polycystin 2 antibody at a dilution of 1:200 in the absence (lens 1 and 2) or presence (lens 3 and 4) of blocking peptide.| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline (PBS), pH 7.4, 1% BSA and 0.05% NaN3. |
