Application
POU4F3 antibody detects POU4F3 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with POU4F3 antibody diluted at 1:1000.Application
POU4F3 antibody detects POU4F3 protein at cytoplasm and nucleus in mouse eye by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. POU4F3 antibody diluted at 1:500.| Product Name | POU4F3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to POU4F3 (POU class 4 homeobox 3)This gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human POU4F3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (98%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DFNA15 BRN3C POU class 4 homeobox 3 |
|---|---|
| Target | POU4F3 |
| Molecular Weight(MW) | 37 kDa (note) |
| Cellular Localization | Nucleus |
Application
POU4F3 antibody detects POU4F3 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with POU4F3 antibody diluted at 1:1000.
Application
POU4F3 antibody detects POU4F3 protein at cytoplasm and nucleus in mouse eye by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. POU4F3 antibody diluted at 1:500.| Positive Control | HeLa nuclear extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
