Application
WB analysis of HepG2 cells using PRAME antibody at 0.2-1μg/ml.Application
WB analysis of K562 cells using PRAME antibody at 1μg/ml.| Product Name | PRAME antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRAMEThis gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide corresponding to a N-terminal region of Human PRAME |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Cow: 92%; Horse: 79%; Human: 100%; Mouse: 92%; Rabbit: 79%; Rat: 92% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MAPE OIP4 PRAME CT130 |
|---|---|
| Target | PRAME |
| Molecular Weight(MW) | 58 KDa (note) |
Application
WB analysis of HepG2 cells using PRAME antibody at 0.2-1μg/ml.
Application
WB analysis of K562 cells using PRAME antibody at 1μg/ml.| Positive Control | HepG2 , K562 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
