Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | AIRE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIRE, APS1, APSI, PGA1, AIRE1, APECED |
| Product description | The function of the protein encoded by the AIRE gene is not well defined. However it contains zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).1) Akiyoshi, H., et al., (2004) J. Biol. Chem. 279 (32), 33984-33991. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AIRE. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| AIRE antibody can be used for detection of AIRE by ELISA at 1:312500. AIRE antibody can be used for detection of AIRE by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AIRE |
|---|---|
| Alternative Names | AIRE APS1 APSI PGA1 AIRE1 APECED |
| Molecular Weight(MW) | 58 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate |
|---|---|
| Application Notes | AIRE antibody can be used for detection of AIRE by ELISA at 1:312500. AIRE antibody can be used for detection of AIRE by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store AIRE antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
