Application
Antibody used in WB on Human HepG2 at 5 ug/ml.| Product Name | AIRE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIRE, APS1, APSI, PGA1, AIRE1, APECED |
| Product description | AIRE is a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).1) Tao, Y., et al., (2006) Mol. Immunol. 43 (4), 335-345. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AIRE. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| AIRE antibody can be used for detection of AIRE by ELISA at 1:1562500. AIRE antibody can be used for detection of AIRE by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AIRE |
|---|---|
| Alternative Names | AIRE APS1 APSI PGA1 AIRE1 APECED |
| Molecular Weight(MW) | 58 kDa |
Application
Antibody used in WB on Human HepG2 at 5 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | AIRE antibody can be used for detection of AIRE by ELISA at 1:1562500. AIRE antibody can be used for detection of AIRE by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store AIRE antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
