Application
Immunohistochemistry staining of AKT1 in skeletal muscle tissue using AKT1 Antibody.Application
Immunohistochemistry staining of AKT1 in tonsil using AKT1 Antibody.| Product Name | AKT1 (phospho Ser473) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA |
| Product description | AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phos-phorylated, AKT dissociates from the membrane and phos-phorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. |
| Immunogen | AKT1 antibody was raised against a peptide located in the phospho C-Terminus aa 460 - 480 of AKT1 (Human). |
| Modification | Phosphoralated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| AKT1 antibody can be used in ELISA starting at 1:000 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT1 |
|---|---|
| Alternative Names | AKT1 AKT AKT-1 C-AKT Pan-AKT PKB alpha PKBalpha Proto-oncogene c-Akt Protein kinase B Rac protein kinase alpha PRKBA Protein kinase B alpha RAC-PK-alpha PKB PKB-ALPHA RAC RAC-ALPHA |
| Tissue Specificity | pSer473 |
Application
Immunohistochemistry staining of AKT1 in skeletal muscle tissue using AKT1 Antibody.
Application
Immunohistochemistry staining of AKT1 in tonsil using AKT1 Antibody.| Application Notes | AKT1 antibody can be used in ELISA starting at 1:000 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store AKT1 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
Data sheet for OM270706