ALG2 is a member of the glycosyltransferase 1 family. It acts as an alpha 1,3 mannosyltransferase, mannosylating Man (2)GlcNAc (2)-dolichol diphosphate and Man (1)GlcNAc (2)-dolichol diphosphate to form Man (3)GlcNAc (2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii).This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man (2)GlcNAc (2)-dolichol diphosphate and Man (1)GlcNAc (2)-dolichol diphosphate to form Man (3)GlcNAc (2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Draeby, I., (2007) Arch. Biochem. Biophys. 467 (1), 87-94.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALG2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ALG2 antibody can be used for detection of ALG2 by ELISA at 1:312500. ALG2 antibody can be used for detection of ALG2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
ALG2 antibody can be used for detection of ALG2 by ELISA at 1:312500. ALG2 antibody can be used for detection of ALG2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ALG2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.