WB
WB analysis using ALG2 Polyclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with ALG2 Polyclonal antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.IHC
IHC staining of human pancreas cancer using ALG2 Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using ALG2 Polyclonal antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | ALG2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ALG2 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:3000 IHC:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALG2 |
|---|---|
| Gene Synonyms | CDG1I CDGIi CMS14 NET38 CMSTA3 hALPG2 |
| Gene Full Name | ALG2 alpha-1,3/1,6-mannosyltransferase |
| Gene Summary | This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008] |
| Alternative Names | ALG2 CDGIi hALPG2 NET38 |
| Molecular Weight(MW) | 22 kDa(Observed band size:47 kDa, 37 kDa) |
| Cellular Localization | Endoplasmic reticulum,Membrane. |
WB
WB analysis using ALG2 Polyclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with ALG2 Polyclonal antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human pancreas cancer using ALG2 Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using ALG2 Polyclonal antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:500-1:3000 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
