Application
Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 μg/mL.Application
Immunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 μg/mL.Application
Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | Apaf1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apaf1 Antibody: CED4, APAF-1, KIAA0413, Apoptotic protease-activating factor 1 |
| Product description | Apaf1 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.1) Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13 |
| Immunogen | Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse. The immunogen is located within the first 50 amino acids of Apa |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 μg/mL. A 115 - 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APAF1 |
|---|---|
| Alternative Names | Apaf1 Antibody: CED4 APAF-1 KIAA0413 Apoptotic protease-activating factor 1 |
| Molecular Weight(MW) | Predicted: 132 kDa Observed: 130 kDa |
| Sequence Similarities | 3) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 μg/mL.
Application
Immunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 μg/mL.
Application
Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 μg/mL. A 115 - 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Apaf1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Apaf1 Antibody is supplied in PBS containing 0.02% sodium azide. |
