Application
Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 0.25 and (B) 0.5 mg/mL.Application
Immunohistochemistry of Apaf1 in K562 cells with Apaf1 antibody at 0.5 μg/mL.| Product Name | Apaf-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apaf-1 Antibody [2E10] : CED4, APAF-1 |
| Product description | Apaf-1 Monoclonal Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.1) Zou H, Henzel WJ, Liu X, et al. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997; 90:405-13. |
| Immunogen | A peptide corresponding to amino acids near the carboxy terminus of human Apaf1. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PWB |
| Apaf1 antibody can be used for detection of Apaf1 by Western blot at 0.25 - 0.5 mg/mL. A 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 0.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APAF1 |
|---|---|
| Alternative Names | Apaf-1 Antibody [2E10] : CED4 APAF-1 |
| Molecular Weight(MW) | 130 kDa |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
Application
Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 0.25 and (B) 0.5 mg/mL.
Application
Immunohistochemistry of Apaf1 in K562 cells with Apaf1 antibody at 0.5 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | Apaf1 antibody can be used for detection of Apaf1 by Western blot at 0.25 - 0.5 mg/mL. A 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 0.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Apaf-1 monoclonal antibody can be stored at -20˚C, stable for one year. |
| Storage Buffer | Apaf-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
