Application
ELISA analysis of control antigen and antigen using PSAP antibody [4D5F4].Application
FACS analysis of HeLa cells using PSAP antibody [4D5F4] (green) or a negative control (purple).Application
ICC/IF analysis of HepG2 cells using PSAP antibody [4D5F4] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
IHC-P analysis of pancreas tissues using PSAP antibody [4D5F4].Application
WB analysis of lysates from (1) untransfected and (2) PSAP (a.a. 325-524)-hIgGFc transfected HEK293 cells using PSAP antibody [4D5F4].Application
WB analysis of human PSAP recombinant protein using PSAP antibody [4D5F4].| Product Name | PSAP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [4D5F4] to PSAPThis gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 325 and 524 of human PSAP expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4D5F4 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | GLBA SAP1 |
|---|---|
| Target | PSAP |
| Molecular Weight(MW) | 58.1 kDa (note) |
Application
ELISA analysis of control antigen and antigen using PSAP antibody [4D5F4].
Application
FACS analysis of HeLa cells using PSAP antibody [4D5F4] (green) or a negative control (purple).
Application
ICC/IF analysis of HepG2 cells using PSAP antibody [4D5F4] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
IHC-P analysis of pancreas tissues using PSAP antibody [4D5F4].
Application
WB analysis of lysates from (1) untransfected and (2) PSAP (a.a. 325-524)-hIgGFc transfected HEK293 cells using PSAP antibody [4D5F4].
Application
WB analysis of human PSAP recombinant protein using PSAP antibody [4D5F4].| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
