Application
Immunofluorescent analysis of PSD95 using Anti-PSD95 Monoclonal Antibody (7E3-1B8) shows staining in C6 Cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 at a dilution of 1:20 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.Application
Immunofluorescent analysis of PSD95 using Anti-PSD95 Monoclonal Antibody (7E3-1B8) shows staining in Hela Cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 at a dilution of 1:20 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.Application
Western blot analysis of PSD95 was performed by loading 25 ug of mouse brain (lane 1) and rat brain (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a PSD95 monoclonal antibody at a dilution of 1:200 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate. Results show a band at ~95 kDa.Application
Flow cytometry analysis of PSD95 in U87-MG cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PSD95 monoclonal antibody at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.| Product Name | PSD95 antibody [7E3-1B8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [7E3-1B8] to PSD95a molecular scaffolding protein that binds and clusters N-methyl-D-aspartate receptors at neuronal synapses; may be involved in guanine nucleotide-mediated signal transduction pathway [RGD, Feb 2006] |
| Immunogen | Purified recombinant rat PSD-95. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7E3-1B8 |
| Host Species | Mouse |
| Tested Applications | BlockingFACSICC/IFIHCIHC-FIPWBChIP |
| ICC/IF: 1:25 - 1:500 | |
| Species Reactivity | HumanMouseChickenRatZebra FishMarsupialsPrimateRabbitXenopusInvertebrates |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | discs, large homolog 4 (Drosophila) , Dlgh4 , Dlg4 , Sap90 |
|---|---|
| Target | PSD95 |
Application
Immunofluorescent analysis of PSD95 using Anti-PSD95 Monoclonal Antibody (7E3-1B8) shows staining in C6 Cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 at a dilution of 1:20 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Application
Immunofluorescent analysis of PSD95 using Anti-PSD95 Monoclonal Antibody (7E3-1B8) shows staining in Hela Cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 at a dilution of 1:20 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Application
Western blot analysis of PSD95 was performed by loading 25 ug of mouse brain (lane 1) and rat brain (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a PSD95 monoclonal antibody at a dilution of 1:200 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate. Results show a band at ~95 kDa.
Application
Flow cytometry analysis of PSD95 in U87-MG cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PSD95 monoclonal antibody at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.| Application Notes | ICC/IF: 1:25 - 1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | PBS containing 0.05 % sodium azide |
