Application
To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.Application
To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.Application
To a sandwich ELISA (assuming 100µl/well), a concentration of 5.0-6.0 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human BAFF when used with ProSci's biotinylated antigen affinity purified anti-human BAFF (XP-5105Bt) as the detection antibody at a concentration of approximately 0.25-0.50 ug/ml.| Product Name | BAFF Antibody [2B] |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The monoclonal antibody was produced in BALB/c mice using recombinant human BAFF as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 5.0-6.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human BAFF when used with our biotinylated antigen affinity purified anti-human BAFF (XP-5105Bt) as the detection antibody at a concentration of approximately 0.25-0.50 μg/mL. Western Blot: To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TNFSF13B |
|---|---|
| Alternative Names | B cell Activating Factor BAFF BLYS TALL1 TNFSF20 ZTNF4 UNQ401/PRO738 Tumor necrosis factor ligand superfamily member 13B B lymphocyte stimulator BLyS DTL CD257 THANK TALL-1 |
Application
To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.
Application
To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.
Application
To a sandwich ELISA (assuming 100µl/well), a concentration of 5.0-6.0 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human BAFF when used with ProSci's biotinylated antigen affinity purified anti-human BAFF (XP-5105Bt) as the detection antibody at a concentration of approximately 0.25-0.50 ug/ml.| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 5.0-6.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human BAFF when used with our biotinylated antigen affinity purified anti-human BAFF (XP-5105Bt) as the detection antibody at a concentration of approximately 0.25-0.50 μg/mL. Western Blot: To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | BAFF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
