Application
Flow cytometry analysis of GCPII (PSMA) by various monoclonal antibodies: GCP-01, GCP-02, GCP-04, GCP-05 on LNCaP cell line. Legend: green column - 1 μg/ml, LNCaP cell line blue column - 5 μg/ml, LNCaP cell line yellow column - negative cell lineApplication
Flow cytometry analysis of GCPII (PSMA) using anti-GCPII (GCP-05) and goat anti-mouse-PE on LNCaP cell line (positive, green) and HeLa cells (negative, blue).| Product Name | PSMA antibody [GCP-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GCP-05] to PSMAGlutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer disease, Huntington disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM? than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.Entrez Gene: 11p11.2 |
| Immunogen | amino acids 44-750 of human GCPII |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GCP-05 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIP |
| Optimal dilutions should be determined experimentally by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FGCP, FOLH, GCP2, GCPII, NAALAD1, NAALAdase, PSM, PSMA, mGCP |
|---|---|
| Target | GCPII |
| Tissue Specificity | The antibody GCP-05 recognizes extracellular domain of glutamate carboxypeptidase II (NAALADase, FOLH1, PSMA), an approximately 95-110 kDa transmembrane glycoprotein expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker. |
Application
Flow cytometry analysis of GCPII (PSMA) by various monoclonal antibodies: GCP-01, GCP-02, GCP-04, GCP-05 on LNCaP cell line. Legend: green column - 1 μg/ml, LNCaP cell line blue column - 5 μg/ml, LNCaP cell line yellow column - negative cell line
Application
Flow cytometry analysis of GCPII (PSMA) using anti-GCPII (GCP-05) and goat anti-mouse-PE on LNCaP cell line (positive, green) and HeLa cells (negative, blue).| Application Notes | Optimal dilutions should be determined experimentally by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
