Mouse monoclonal [25D5] antibody to PSMC5Human PSMC5 (also known as SUG1, p45, Rpt6 and Cim3) is a component of the 19S regulatory subunit of the 26S proteosome complex. It is one of the AAA ATPas found in the 19S regulatory complex that is thought to participate in the unfolding of ubiquitinated proteins in an ATP-dependent manner. It has also been shown to directly interact with the nucleotide excision repair protein XPB.
Immunogen
Recombinant protein encoding full-length SUG1 purified from E. coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG
Clone Number
25D5
Host Species
Mouse
Tested Applications
WB
For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
S8 SUG-1 SUG1 TBP10 TRIP1 p45 p45/SUG SUG 1
Target
SUG1
Molecular Weight(MW)
45 kDa (note)
Cellular Localization
Cytoplasmic
Application
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 D: Hela E: Hep G2 F: Molt-4 G: Raji 10% SDS PAGE diluted at 1:1000
Positive Control
HeLa
Application Notes
For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Storage Buffer
Phosphate-buffered saline, pH 7.4, containing no preservatives