Application
Immunohistochemistry of caspase-13 in mouse brain tissue with caspase-13 antibody at 10 μg/mL.Application
Western blot analysis of caspase-13 in human HL60 cell lysate (A), mouse brain (B) and rat brain (C) tissue lysates with caspase-13 antibody at 1 μg/mL.| Product Name | Caspase-13 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Caspase-13 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death is finally caused by members of the caspase family of proteases and caspase activated DNases. A novel member in the caspase family was recently identified and designated ERICE (for Evolutionarily Related Interleukin-1 beta Converting Enzyme) and caspase-13. Caspase-13 belongs to the ICE subfamily of caspases. Overexpression of caspase-13 induces apoptosis. Caspase-13 was activated by caspase-8, which is a key enzyme in death receptor induced apoptosis. Caspase-13 is expressed in a variety of human tissues and cell lines.1) Humke EW, Ni J, Dixit VM. ERICE, a novel FLICE-activatable caspase. J Biol Chem 1998;273:15702-7 (WD0500) |
| Immunogen | Caspase-13 antibody was raised against a 15 amino acid peptide near the amino terminus of human Caspase-13. The immunogen is located within amino acids 80 - 130 of Caspase-13. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| Caspase-13 antibody can be used for detection of caspase-13 by Western blot at 1 μg/mL. A 43 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP4 |
|---|---|
| Alternative Names | Caspase-13 Antibody: CASP13 Caspase-13 Evolutionary related interleukin-1-beta-converting enzyme CASP-13 |
| Molecular Weight(MW) | 43 kDa |
Application
Immunohistochemistry of caspase-13 in mouse brain tissue with caspase-13 antibody at 10 μg/mL.
Application
Western blot analysis of caspase-13 in human HL60 cell lysate (A), mouse brain (B) and rat brain (C) tissue lysates with caspase-13 antibody at 1 μg/mL.| Application Notes | Caspase-13 antibody can be used for detection of caspase-13 by Western blot at 1 μg/mL. A 43 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-13 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Caspase-13 Antibody is supplied in PBS containing 0.02% sodium azide. |
