Application
Immunofluorescence of Caspase-4 in A20 cells with Caspase-4 antibody at 10 μg/mL.Application
Immunohistochemical staining of human spleen tissue using caspase-4 antibody at 2 μg/mL.Application
Western blot analysis of caspase-4 in human spleen cells with caspase-4 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Caspase-4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Caspase-4 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells. More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease.1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74. |
| Immunogen | Caspase-4 antibody was raised against a 15 amino acid synthetic peptide from near the center of human Caspase-4. The immunogen is located within amino acids 100 - 150 of Caspase-4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF |
| Casp-4 antibody can be used for the detection of Caspase-4 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP4 |
|---|---|
| Alternative Names | Caspase-4 Antibody: TX ICH-2 Mih1/TX ICEREL-II ICE(rel)II ICH2 Caspase-4 Protease ICH-2 CASP-4 |
Application
Immunofluorescence of Caspase-4 in A20 cells with Caspase-4 antibody at 10 μg/mL.
Application
Immunohistochemical staining of human spleen tissue using caspase-4 antibody at 2 μg/mL.
Application
Western blot analysis of caspase-4 in human spleen cells with caspase-4 antibody at (A) 1 and (B) 2 μg/mL.| Application Notes | Casp-4 antibody can be used for the detection of Caspase-4 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Caspase-4 Antibody is supplied in PBS containing 0.02% sodium azide. |
