Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Immunofluorescence staining of methanol-fixed HepG2 cells using CD227/MUC1 (Phospho-Tyr1229) Antibody.Application
Western blot analysis of extracts from HepG2 cells treated with PMA using CD227/MUC1 (Phospho-Tyr1229) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | CD227/MUC1 (phospho Tyr1229) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PUM, EMA, H23AG, MUC-1, PEM |
| Product description | The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.1) Lan M.S., J. Biol. Chem. 265:15294-15299(1990). |
| Immunogen | CD227/MUC1 (phospho Tyr1229) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 1229 S-P-Y (p)-E-K) derived from Human CD227/MUC1. |
| Modification | p-Tyr1229 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MUC1 |
|---|---|
| Alternative Names | PUM EMA H23AG MUC-1 PEM |
| Molecular Weight(MW) | 170 kDa |
| Tissue Specificity | The antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at tyrosine 1229. |
| Entrez Gene | 4582 |
|---|---|
| Protein Accession | NP_001018016.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Immunofluorescence staining of methanol-fixed HepG2 cells using CD227/MUC1 (Phospho-Tyr1229) Antibody.
Application
Western blot analysis of extracts from HepG2 cells treated with PMA using CD227/MUC1 (Phospho-Tyr1229) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
