Application
Western blot analysis using CD31 monoclonal antibody against truncated CD31 recombinant protein.Application
Immunohistochemical analysis of paraffin - embedded human lymphonodus tissue showing cytoplasmic location of vascular endothelial cells using CD31 antibody with DAB staining.| Product Name | CD31 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EndoCAM, PECAM-1, CD31, PECA1, GPIIA', endoCAM, CD31/EndoCAM |
| Product description | CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors.It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells.The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1.It is also weakly expressed on many peripheral lymphoid cells and platelets.CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).1) Mayr U et al. Circ Res 98:412-20 (2006). |
| Immunogen | Ni-NTA purified recombinant CD31 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PECAM1 |
|---|---|
| Alternative Names | EndoCAM PECAM-1 CD31 PECA1 GPIIA' endoCAM CD31/EndoCAM |
Application
Western blot analysis using CD31 monoclonal antibody against truncated CD31 recombinant protein.
Application
Immunohistochemical analysis of paraffin - embedded human lymphonodus tissue showing cytoplasmic location of vascular endothelial cells using CD31 antibody with DAB staining.| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CD31 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
