Application
Immunofluorescense analysis of peripheral blood cells using CD34 monoclonal antibody with FITC - IgG.Application
Western blot analysis using CD34 monoclonal antibody against truncated CD34 recombinant protein.| Product Name | CD34 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hematopoietic progenitor cell antigen CD34, CD_antigen=CD34 |
| Product description | CD34 is a transmembrane glycoprotein with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells, endothelial cells and some fibroblasts. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells. CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.1) Sho E., et al. Arterioscler Thromb Vasc Biol. 2004: 24: 1916-21. |
| Immunogen | Ni-NTA purified truncated recombinant CD34 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAIFWB |
| Dilution Western Blot:1:200 - 1:1,000 IF:1:25 - 1:100 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CD34 |
|---|---|
| Alternative Names | Hematopoietic progenitor cell antigen CD34 CD_antigen=CD34 |
Application
Immunofluorescense analysis of peripheral blood cells using CD34 monoclonal antibody with FITC - IgG.
Application
Western blot analysis using CD34 monoclonal antibody against truncated CD34 recombinant protein.| Positive Control | 1) Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate |
|---|---|
| Application Notes | Dilution Western Blot:1:200 - 1:1,000 IF:1:25 - 1:100 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CD34 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
