CFLAR might contribute to the carcinogenesis and aggressiveness of endometrial carcinoma and might be a useful prognostic factor in the tumor. CFLAR is specially overexpressed in colon cancers and it might contribute to carcinogenesis of normal colonic mucosa.1) Kondo, G., et al., (2005) Oncol Rep. 14(5), 1215-22.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CFLAR.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
CFLAR antibody can be used for detection of CFLAR by ELISA at 1:312500. CFLAR antibody can be used for detection of CFLAR by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.
Application
Antibody used in WB on Human 293T at: 1:1000.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
CFLAR antibody can be used for detection of CFLAR by ELISA at 1:312500. CFLAR antibody can be used for detection of CFLAR by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CFLAR antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt