Application
Immunohistochemistry staining of CFLAR in skeletal muscle tissue using CFLAR Antibody.| Product Name | CFLAR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CFLAR, C-FLIPR, C-FLIPS, C-FLIP, CASP8AP1, Cflip, CLARP, CASH, Caspase homolog, Caspase-eight-related protein, Flice inhibitory protein, I-FLICE, Inhibitor of FLICE, FLAME, FLAME1, MRIT, Usurpin beta, C-FLIPL, Casper, FLAME-1, FLIP, Usurpin |
| Product description | I-FLICE (inhibitor of FLICE) is a catalytically inactive form FLICE (Caspase 8) and Mch4/FLICE2 (Caspase 10). I-FLICE shares sequence homologies to FLICE and Mch4/FLICE2 and binds to these two molecules as demonstrated by co-immunoprecipitation anlalysis. However, unlike the effector proteases FLICE and Mch4/FLICE2, I-FLICE does not bind to the adaptor molecule FADD. I-FLICE inhibits both TNFR-1 and CD-95 induced apoptosis. Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPa, b, g, and d, respectively. |
| Immunogen | A peptide corresponding to amino acids 206-222 GRSKEQRLKEQLGAQQE of human I-FLICE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| CFLAR antibody can be used in ELISA, Western Blot, and immunohistochemistry starting at 5 μg/mL.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | CFLAR |
|---|---|
| Alternative Names | CFLAR C-FLIPR C-FLIPS C-FLIP CASP8AP1 Cflip CLARP CASH Caspase homolog Caspase-eight-related protein Flice inhibitory protein I-FLICE Inhibitor of FLICE FLAME FLAME1 MRIT Usurpin beta C-FLIPL Casper FLAME-1 FLIP Usurpin |
Application
Immunohistochemistry staining of CFLAR in skeletal muscle tissue using CFLAR Antibody.| Application Notes | CFLAR antibody can be used in ELISA, Western Blot, and immunohistochemistry starting at 5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CFLAR antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.05% BSA, 0.05% sodium azide. |
