Application
Western Blot at 1:1000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | COL2A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Collagen alpha-1(II) chain, Alpha-1 type II collagen, Collagen alpha-1(II) chain, Chondrocalcin, COL2A1 |
| Product description | Alpha-1 chain of type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutant forms of this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.1) Olavarrieta,L., Clin. Genet. 73 (3), 262-267 (2008) |
| Immunogen | This COL2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1209-1237 amino acids from the C-terminal region of human COL2A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL2A1 |
|---|---|
| Alternative Names | Collagen alpha-1(II) chain Alpha-1 type II collagen Collagen alpha-1(II) chain Chondrocalcin COL2A1 |
| Molecular Weight(MW) | 142 kDa |
Application
Western Blot at 1:1000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
