Application
Western blot analysis using CK monoclonal antibody against truncated CK recombinant protein.Application
Immunohistochemical analysis of paraffin - embedded human colon adenocarcinoma (top) showing cytoplasma / membrane location using CK antibody with DAB staining. Imunofluorescense staining of methanol - fixed HepG - 2 cells (bottom) showing cytoplasm localization.| Product Name | Cytokeratin Antibody [7H8C4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors.1) Scope A. Schwendenwein I. Frommlet F. Vet Rec. 2006, Dec 16, 159(25): 839-43. |
| Immunogen | Ni-NTA purified truncated recombinant CK expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | KRT5 |
|---|---|
| Alternative Names | 58 kDa cytokeratin CK-5 K5 CK5 DDD DDD1 EBS2 KRT5A |
Application
Western blot analysis using CK monoclonal antibody against truncated CK recombinant protein.
Application
Immunohistochemical analysis of paraffin - embedded human colon adenocarcinoma (top) showing cytoplasma / membrane location using CK antibody with DAB staining. Imunofluorescense staining of methanol - fixed HepG - 2 cells (bottom) showing cytoplasm localization.| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Cytokeratin(Pan) monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
Data sheet for OM276672