Application
Immunohistochemical analysis of paraffin - embedded human normal esophagus epithelium (top) and human human normal salivary gland basal cell (bottom) showing cytoplasma / membrane location using CK5 antibody with DAB staining.Application
Western blot analysis using CK5 monoclonal antibody against truncated CK5 - Trx - His recombinant protein| Product Name | Cytokeratin5 Antibody [3E2F1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CK5 (keratin 5) is a member of the keratin gene family. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin5 gene (KRT5) have been shown to result in the autosomal dominant disorderepidermolysis bullosa (EB). Defects in KRT5 are a cause of epidermolysis bullosa simplex.1) Canedo-Patzi AM. Leon-Bojorge B. de Ortiz-Hidalgo C. Gac Med Mex. 2006, Jan-Feb, 142(1):59-66. Spanish. |
| Immunogen | Ni-NTA purified truncated recombinant CK5-Trx-His expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | KRT5 |
|---|---|
| Alternative Names | 58 kDa cytokeratin CK-5 K5 CK5 DDD DDD1 EBS2 KRT5A |
Application
Immunohistochemical analysis of paraffin - embedded human normal esophagus epithelium (top) and human human normal salivary gland basal cell (bottom) showing cytoplasma / membrane location using CK5 antibody with DAB staining.
Application
Western blot analysis using CK5 monoclonal antibody against truncated CK5 - Trx - His recombinant protein| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Cytokeratin 5 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
Data sheet for OM276674