Application
Western blot analysis of (1) 20 ng of DDDDK-tagged recombinant GGP1 protein and (2) 20 ng of DDDDK-tagged recombinant GGP1 protein using 1 μg of DDDDK-tag antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.Application
Western blot analysis of (1) 1000ng, (2) 500 ng, (3) 250 ng, and (4) 125 ng of a DDDDK-tag-containing recombinant GGP1 protein with DDDDK-tag antibody at 0.5 μg/ml.| Product Name | DDDDK-tag Antibody [1D1B12] |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. DDDDK-tag is one of the more popular epitope tags for expressed recombinant proteins. |
| Immunogen | DDDDK-tag antibody was raised against a synthetic peptide containing DYKDDDDK. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAIPWB |
| DDDDK-tag antibody can be used for detection of DDDDK-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml. | |
| Species Reactivity | Tag |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | N/A |
|---|---|
| Alternative Names | DDDDK-tag DDDDK DDDDK-tag DDDDK flag-tag flag |
| Molecular Weight(MW) | N/A |
| Tissue Specificity | DDDDK-tag antibody will detect amino- or carboxy-terminal DDDDK-tagged recombinant proteins. |
Application
Western blot analysis of (1) 20 ng of DDDDK-tagged recombinant GGP1 protein and (2) 20 ng of DDDDK-tagged recombinant GGP1 protein using 1 μg of DDDDK-tag antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.
Application
Western blot analysis of (1) 1000ng, (2) 500 ng, (3) 250 ng, and (4) 125 ng of a DDDDK-tag-containing recombinant GGP1 protein with DDDDK-tag antibody at 0.5 μg/ml.| Positive Control | 1) N/A |
|---|---|
| Application Notes | DDDDK-tag antibody can be used for detection of DDDDK-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml. |
| Form | Liquid |
|---|---|
| Storage Instructions | DDDDK-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | DDDDK-tag antibody is supplied in PBS containing 0.02% sodium azide. |
