Application
DIO2 Antibody IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of DIO2 Antibody in MCF-7 cell line lysates (35ug/lane)Application
Western blot analysis of lysates from human heart and human skeletal muscle tissue lysate (from left to right), using DIO2 Antibody at 1:1000 at each lane.| Product Name | DIO2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Type II iodothyronine deiodinase, 5DII, DIOII, Type 2 DI, Type-II 5'-deiodinase, DIO2, ITDI2, TXDI2 |
| Product description | DIO2 belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3).1) He,B.,et.al., Prog. Neuropsychopharmacol. Biol. Psychiatry 33 (6), 986-990 (2009) |
| Immunogen | This DIO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-191 amino acids from the Central region of human DIO2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DIO2 |
|---|---|
| Alternative Names | Type II iodothyronine deiodinase 5DII DIOII Type 2 DI Type-II 5'-deiodinase DIO2 ITDI2 TXDI2 |
| Molecular Weight(MW) | 31 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig |
Application
DIO2 Antibody IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of DIO2 Antibody in MCF-7 cell line lysates (35ug/lane)
Application
Western blot analysis of lysates from human heart and human skeletal muscle tissue lysate (from left to right), using DIO2 Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
