WB
MCF-7 cells were subjected to SDS PAGE followed by western blot with DIO2 Antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using DIO2 Antibody at dilution of 1:200 (under 10x lens).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIO2 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Product Name | DIO2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human DIO2 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:100-1:400 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.7mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DIO2 |
|---|---|
| Gene Synonyms | D2 5DII SelY DIOII TXDI2 SELENOY |
| Gene Full Name | iodothyronine deiodinase 2 |
| Gene Summary | The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This gene is widely expressed, including in thyroid and brain. It is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues. It has also been reported to be highly expressed in thyroids of patients with Graves disease, and in follicular adenomas. The intrathyroidal T4 to T3 conversion by this enzyme may contribute significantly to the relative increase in thyroidal T3 production in these patients. This protein is a selenoprotein containing the non-standard amino acid, selenocysteine (Sec), which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Unlike the other two members (DIO1 and DIO3) of this enzyme family, the mRNA for this gene contains an additional in-frame UGA codon that has been reported (in human) to function either as a Sec or a stop codon, which can result in two isoforms with one or two Sec residues; however, only the upstream Sec (conserved with the single Sec residue found at the active site in DIO1 and DIO3) was shown to be essential for enzyme activity (PMID:10403186). Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2018] |
| Alternative Names | 5DII D2 DIOII EC:1.21.99.4 ITDI2 |
| Molecular Weight(MW) | 35-40 kDa |
| Cellular Localization | Endoplasmic reticulum membrane. |
WB
MCF-7 cells were subjected to SDS PAGE followed by western blot with DIO2 Antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using DIO2 Antibody at dilution of 1:200 (under 10x lens).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIO2 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Application Notes | WB:1:500-1:1000 IHC:1:100-1:400 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
