Application
Immunohistochemistry of DR4 in human spleen tissue with DR4 antibody at 5 μg/mL.Application
Immunofluorescence of DR3 in human spleen tissue with DR3 antibody at 20 μg/ml.Application
Immunocytochemistry of DR4 in Jurkat cells with DR4 antibody at 10 μg/mL.Application
Western blot analysis of DR4 in (A) HepG2 and (B) Jurkat cell lysate with DR4 antibody at 1 μg/ml.| Product Name | DR4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DR4 Antibody: DR4, APO2, CD261, TRAILR1, TRAILR-1, DR4, Tumor necrosis factor receptor superfamily member 10A, Death receptor 4, TRAIL receptor 1 |
| Product description | DR4 Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4). The ligand for this novel death receptor has been identified and termed TRAIL2, 3, which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1). DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-κB activation in many tissues and cells.1) Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J; Dixit VM. The receptor for the cytotoxic ligand TRAIL. Science; 1997;276:111-113 |
| Immunogen | DR4 antibody was raised against a peptide corresponding to 19 amino acids near the carboxy terminus of human DR4 protein. The immunogen is located within the last 50 amino acids of DR4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIHC-PWB |
| DR4 antibody can be used for detection of DR4 expression by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFRSF10A |
|---|---|
| Alternative Names | DR4 Antibody: DR4 APO2 CD261 TRAILR1 TRAILR-1 DR4 Tumor necrosis factor receptor superfamily member 10A Death receptor 4 TRAIL receptor 1 |
| Molecular Weight(MW) | Predicted: 56 kDa Observed: 56 kDa |
| Sequence Similarities | 4) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Immunohistochemistry of DR4 in human spleen tissue with DR4 antibody at 5 μg/mL.
Application
Immunofluorescence of DR3 in human spleen tissue with DR3 antibody at 20 μg/ml.
Application
Immunocytochemistry of DR4 in Jurkat cells with DR4 antibody at 10 μg/mL.
Application
Western blot analysis of DR4 in (A) HepG2 and (B) Jurkat cell lysate with DR4 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | DR4 antibody can be used for detection of DR4 expression by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DR4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DR4 Antibody is supplied in PBS containing 0.02% sodium azide. |
