Application
Western blot analysis of DR4 in HeLa (H), K562 (K), and Jurkat (J) whole cell lysate with DR4 antibody at 1:500 dilution.| Product Name | DR4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DR4 Antibody: DR4, APO2, CD261, TRAILR1, TRAILR-1, DR4, Tumor necrosis factor receptor superfamily member 10A, Death receptor 4, TRAIL receptor 1 |
| Product description | DR4 Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4). The ligand for this novel death receptor has been identified and termed TRAIL, which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1). DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-κB activation in many tissues and cells.1) Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J; Dixit VM. The receptor for the cytotoxic ligand TRAIL. Science; 1997;276:111-113 |
| Immunogen | DR4 antibody was raised against a 20 amino acid peptide near the amino terminus of human DR4. The immunogen is located within the first 50 amino acids of DR4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DR4 antibody can be used for detection of DR4 by Western blot 0.5 μg/mL. A 57 kDa band can be detected.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFRSF10A |
|---|---|
| Alternative Names | DR4 Antibody: DR4 APO2 CD261 TRAILR1 TRAILR-1 DR4 Tumor necrosis factor receptor superfamily member 10A Death receptor 4 TRAIL receptor 1 |
| Molecular Weight(MW) | 57 kDa |
| Sequence Similarities | 2) Cat. No. 1205 - Jurkat Cell Lysate |
| Tissue Specificity | DR4 antibody has no cross reaction to DR5. |
Application
Western blot analysis of DR4 in HeLa (H), K562 (K), and Jurkat (J) whole cell lysate with DR4 antibody at 1:500 dilution.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | DR4 antibody can be used for detection of DR4 by Western blot 0.5 μg/mL. A 57 kDa band can be detected.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DR4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DR4 Antibody is supplied in PBS containing 0.02% sodium azide. |
