Application
Formalin-fixed and paraffin-embedded human lung carcinoma with Fascin Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of NCI-H292 cells using Fascin Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of Fascin Antibody in Y79 cell line lysates (35ug/lane)| Product Name | Fascin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Fascin organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. It is probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers.1) Qualtrough,D., Br. J. Cancer 101 (7), 1124-1129 (2009) |
| Immunogen | This Fascin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from the Central region of human Fascin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | FSCN1 |
|---|---|
| Alternative Names | Fascin 55 kDa actin-bundling protein Singed-like protein p55 FSCN1 FAN1 HSN SNL |
| Molecular Weight(MW) | 55 kDa |
Application
Formalin-fixed and paraffin-embedded human lung carcinoma with Fascin Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of NCI-H292 cells using Fascin Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of Fascin Antibody in Y79 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
