WB
HeLa cells were subjected to SDS PAGE followed by western blot with Fascin Antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Fascin Antibody at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using Fascin antibody at dilution of 1:50 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).IF-P
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Fascin antibody at dilution of 1:400 and Omnimabs® 488 Goat Ant-Mouse IgG(H+L).FC
1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Fascin Monoclonal antibody and Omnimabs® 488 Goat Anti-Mouse IgG(H+L)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | Fascin Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human Fascin protein |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:4000 IHC:1:20-1:2000 ICC/IF:1:50-1:500 IF-P:1:200-1:800 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | Human |
| Concentration | 2.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FSCN1 |
|---|---|
| Gene Synonyms | HSN SNL p55 FAN1 |
| Gene Full Name | fascin actin-bundling protein 1 |
| Gene Summary | This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011] |
| Alternative Names | FSCN1 1B5G3 55 kDa actin bundling protein 55 kDa actin-bundling protein Fascin 1 Fascin |
| Molecular Weight(MW) | 55kDa |
| Cellular Localization | Cell junction,Cell projection,Cytoplasm,Cytoskeleton. |
WB
HeLa cells were subjected to SDS PAGE followed by western blot with Fascin Antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Fascin Antibody at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using Fascin antibody at dilution of 1:50 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).
IF-P
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Fascin antibody at dilution of 1:400 and Omnimabs® 488 Goat Ant-Mouse IgG(H+L).
FC
1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Fascin Monoclonal antibody and Omnimabs® 488 Goat Anti-Mouse IgG(H+L)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:1000-1:4000 IHC:1:20-1:2000 ICC/IF:1:50-1:500 IF-P:1:200-1:800 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
