Application
Top Image: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using FKHR (Phospho-Ser256). Bottom Image: Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR (Phospho-Ser256).Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using FKHR (Phospho-Ser256).| Product Name | FKHR (phospho Ser256) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Transcription factor.1) Gan L, et al. (2005) J Neurochem; 93 (5): 1209-19. Smith WW, et al. (2005) J Cell Biol; 169 (2): 331-9. Di Maira G, et al. (2005) Cell Death Differ; 12 (6): 668-77. Horn S, et al. (2004) Leukemia; 18 (11): 1839-49. |
| Immunogen | FKHR (Phospho-Ser256) antibody was raised against a peptide sequence around phosphorylation site of serine 256 (A-A-S (p) -M-D) derived from Human FKHR. |
| Modification | p-Ser256 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXO1 |
|---|---|
| Alternative Names | FKH1 FKHR FOXO1A CSBP CSBP1 CSBP2 CSPB1 MXI2 SAPK2A FOXO1 |
| Molecular Weight(MW) | 78 - 82 kDa |
| Tissue Specificity | This antibody detects endogenous level of FKHR only when phosphorylated at serine 256. |
| Entrez Gene | 2308 |
|---|---|
| Protein Accession | NP_002006.2 |
Application
Top Image: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using FKHR (Phospho-Ser256). Bottom Image: Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR (Phospho-Ser256).
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using FKHR (Phospho-Ser256).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
