Application
Immunocytochemistry of FOXO1 in HeLa cells with FOXO1 antibodyApplication
Western blot analysis of FOXO1 in Hela cell lysate with FOXO1 antibody at 1 μg/mL.| Product Name | FOXO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FOXO1 Antibody: FKH1, FKHR, FOXO1A, Forkhead box protein O1, Forkhead box protein O1A |
| Product description | FOXO1 Antibody: FOXO1, also known as FKHR or ForkHead in Rhabdomyosarcoma, is a 70 kDa protein which is a ubiquitously expressed member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In insulin-responsive tissues, stress or nutrient abundance triggers phosphorylation by PKB/AKT, blocking nuclear translocation and activity. Genetic mutations involving FOXO1A are a cause of alveolar rhabdomyosarcoma (RMS2). Recent studies link the anti-tumor activity of FOXO1 and the process of autophagy.1) Anderson MJ, Viars CS, Czekay S, et al. Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics1998; 47:187-99. |
| Immunogen | FOXO1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human FOXO1. The immunogen is located within the first 50 amino acids of FOXO1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| WB:1:500~1:1000 ICC/IF:1:100~1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXO1 |
|---|---|
| Alternative Names | FOXO1 Antibody: FKH1 FKHR FOXO1A Forkhead box protein O1 Forkhead box protein O1A |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of FOXO1 in HeLa cells with FOXO1 antibody
Application
Western blot analysis of FOXO1 in Hela cell lysate with FOXO1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:1000 ICC/IF:1:100~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | FOXO1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FOXO1 Antibody is supplied in PBS containing 0.02% sodium azide. |
