Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR (Phospho-Ser319). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using FKHR (Phospho-Ser319).Application
Western blot analysis of lysed extracts from 3T3 cells untreated or treated with serum starvation using FKHR (Phospho-Ser319).| Product Name | FKHR (phospho Ser319) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FKHR belongs to the forkhead family of transcription factors, which are characterized by a distinct forkhead domain. It may play a role in myogenic growth and differentiation. The mammalian DAF-16-like transcription factors, FKHR, FKHRL1, and AFX, function as key regulators of insulin signaling, cell cycle progression, and apoptosis downstream of phosphoinositide 3-kinase. Gene activation through binding to insulin response sequences has been essential for mediating these functions. D-type Cyclins (in Class III) is required for FKHR mediated inhibition of cell cycle progression and transformation. FKHR gene is mapped to chromosome 13q141) Rena G, et al. (2002) EMBO J 21 (9): 2263-2271. Woods YL, et al. (2001) Biochem J355 (Pt 3): 597-607. Rena G, et al. (2001) Biochem J 354 (Pt 3): 605-612. |
| Immunogen | FKHR (Phospho-Ser319) antibody was raised against a peptide sequence around phosphorylation site of serine 319 (T-S-S (p) -N-A) derived from Human FKHR/FOXO1A. |
| Modification | p-Ser319 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXO1 |
|---|---|
| Alternative Names | FKH1 FKHR FOXO1A MEK2 MKK2 PRKMK2 FOXO1 |
| Molecular Weight(MW) | 78 - 82 kDa |
| Tissue Specificity | This antibody detects endogenous level of FKHR only when phosphorylated at serine 319. |
| Entrez Gene | 2308 |
|---|---|
| Protein Accession | NP_002006.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR (Phospho-Ser319). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using FKHR (Phospho-Ser319).
Application
Western blot analysis of lysed extracts from 3T3 cells untreated or treated with serum starvation using FKHR (Phospho-Ser319).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
