Application
Immunohistochemical staining of GAPDH in HeLa cells at a dilution of 10 μg/mL.Application
Western blot analysis of GAPDH in A431, Daudi, HepG2, HL60, Jurkat, Human kidney, Mouse lung, and Chicken liver lysate with GAPDH antibody 0.5 μg/mL.Application
Immunofluorescence of GAPDH in HeLa cells with GAPDH antibody at 20 μg/mL.| Product Name | GAPDH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GAPDH Antibody: Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD |
| Product description | GAPDH Antibodies (anti-GAPDH antibody): Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. GAPDH antibodies have also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. The Anti- GAPDH antibody is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.1) Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52. |
| Immunogen | GAPDH antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human GAPDH. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCWB |
| GAPDH antibody can be used for the detection of GAPDH by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GAPDH |
|---|---|
| Alternative Names | GAPDH Antibody: Glyceraldehyde-3-phosphate dehydrogenase G3PDH GAPD |
Application
Immunohistochemical staining of GAPDH in HeLa cells at a dilution of 10 μg/mL.
Application
Western blot analysis of GAPDH in A431, Daudi, HepG2, HL60, Jurkat, Human kidney, Mouse lung, and Chicken liver lysate with GAPDH antibody 0.5 μg/mL.
Application
Immunofluorescence of GAPDH in HeLa cells with GAPDH antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | GAPDH antibody can be used for the detection of GAPDH by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GAPDH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GAPDH Antibody is supplied in PBS containing 0.02% sodium azide. |
