Application
Western blot analysis of GAPDH in multiple cell and tissue lysates with GAPDH antibody at 1 μg/ml. Lanes 1-23, 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, rabbit brain, human heart, rat heart, human liver, mouse liver, rat liver, rabbit liver, respectively.| Product Name | GAPDH Antibody [12D3D5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | 1) Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52. |
| Immunogen | GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GAPDH |
|---|---|
| Alternative Names | Glyceraldehyde-3-phosphate dehydrogenase G3PDH GAPD |
| Molecular Weight(MW) | Predicted: 37 kDa Observed: 36 kDa |
| Tissue Specificity | GAPDH antibody is human, mouse,and rat reactive. |
Application
Western blot analysis of GAPDH in multiple cell and tissue lysates with GAPDH antibody at 1 μg/ml. Lanes 1-23, 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, rabbit brain, human heart, rat heart, human liver, mouse liver, rat liver, rabbit liver, respectively.| Application Notes | GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | GAPDH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | GAPDH antibody is supplied in PBS containing 0.02% sodium azide. |
