Application
Immunohistochemistry of GAPDH in human liver tissue with GAPDH antibodyApplication
Immunofluorescence of GAPDH in HeLa cells with GAPDH antibody at 10 μg/mL.Application
Western blot analysis of GAPDH in HeLa cell lysate with GAPDH antibody at (A) 0.125, (B) 0.25 and (C) 1 μg/mL.| Product Name | GAPDH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GAPDH Antibody: G3PD, GAPD, HEL-S-162eP, CDABP0047, OK/SW-cl.12, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH |
| Product description | GAPDH Antibody: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.1) Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52. |
| Immunogen | GAPDH antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human GAPDH. The immunogen is located within amino acids 60 - 110 of GAPDH. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WB:1:500~1:2000 IF:1:100~1:500 IHC-p:1:50~1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GAPDH |
|---|---|
| Alternative Names | GAPDH Antibody: G3PD GAPD HEL-S-162eP CDABP0047 OK/SW-cl.12 Glyceraldehyde-3-phosphate dehydrogenase Peptidyl-cysteine S-nitrosylase GAPDH GAPDH |
| Sequence Similarities | 3) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunohistochemistry of GAPDH in human liver tissue with GAPDH antibody
Application
Immunofluorescence of GAPDH in HeLa cells with GAPDH antibody at 10 μg/mL.
Application
Western blot analysis of GAPDH in HeLa cell lysate with GAPDH antibody at (A) 0.125, (B) 0.25 and (C) 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IF:1:100~1:500 IHC-p:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | GAPDH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GAPDH Antibody is supplied in PBS containing 0.02% sodium azide. |
