Application
Western blot analysis of GAPDH in multiple cell and tissue lysates with GAPDH antibody at 1 μg/ml. Lanes 1-23, 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, rabbit brain, human heart, rat heart, human liver, mouse liver, rat liver, rabbit liver, respectively.WB
A Representative Western blotting bands (a) showing the effect of OT and GYY4137 on GFAP expression in the SON in brain slices and the summary graphs.| Product Name | GAPDH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.1) Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52. |
| Immunogen | GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH. The immunogen is located within amino acids 230 - 280 of GAPDH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GAPDH |
|---|---|
| Alternative Names | Glyceraldehyde-3-phosphate dehydrogenase G3PDH GAPD |
| Molecular Weight(MW) | Predicted: 37 kDa Observed: 36 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (100%) |
| Tissue Specificity | GAPDH antibody is human, mouse,and rat reactive. |
Application
Western blot analysis of GAPDH in multiple cell and tissue lysates with GAPDH antibody at 1 μg/ml. Lanes 1-23, 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, rabbit brain, human heart, rat heart, human liver, mouse liver, rat liver, rabbit liver, respectively.
WB
A Representative Western blotting bands (a) showing the effect of OT and GYY4137 on GFAP expression in the SON in brain slices and the summary graphs.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GAPDH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | GAPDH antibody is supplied in PBS containing 0.02% sodium azide. |
