Application
Western blot analysis of extract from JK, HL-60, HUVEC and HeLa cells using GAPDH Antibody.| Product Name | GAPDH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.1) Ercolani L., Florence B., Denaro M., Alexander M. J. Biol. Chem. 263:15335-15341 (1988) Tisdale E. J. J. Biol. Chem. 277:3334-3341 (2002) |
| Immunogen | GAPDH antibody was raised against a peptide sequence around aa.252~256 (P-A-K-Y-D) derived from Human GAPDH. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GAPDH |
|---|---|
| Alternative Names | G3PD GAPD HEL-S-162eP G3PD MGC88685 |
| Molecular Weight(MW) | 37 kDa |
| Tissue Specificity | This antibody detects endogenous level of total GAPDH protein. |
| Entrez Gene | 2597 |
|---|---|
| Protein Accession | NP_002037.2 |
Application
Western blot analysis of extract from JK, HL-60, HUVEC and HeLa cells using GAPDH Antibody.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
