Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 ug/ml–0.150 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vaApplication
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 ug/ml–0.150 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vaApplication
To detect Human GRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.| Product Name | GRO/MGSA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FSP, GRO1, GROa, MGSA, NAP-3, SCYB1, MGSA-a, GRO, GROA, Growth-regulated alpha protein, C-X-C motif chemokine 1 |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGRO/MGSA (human GRO/MGSA). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | Neut |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGRO/MGSA 100 ng/mL), a concentration of 1.5 - 3.0 μg/mL of this antibody is required. ELISA: To detect hGRO/MGSA by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGRO/MGSA. Sandwich: To detect hGRO/MGSA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human GRO/MGSA (XP-5147Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGRO/MGSA. Western Blot: To detect hGRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO/MGSA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXCL1 |
|---|---|
| Alternative Names | FSP GRO1 GROa MGSA NAP-3 SCYB1 MGSA-a GRO GROA Growth-regulated alpha protein C-X-C motif chemokine 1 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 ug/ml–0.150 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may va
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 ug/ml–0.150 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may va
Application
To detect Human GRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGRO/MGSA 100 ng/mL), a concentration of 1.5 - 3.0 μg/mL of this antibody is required. ELISA: To detect hGRO/MGSA by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGRO/MGSA. Sandwich: To detect hGRO/MGSA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human GRO/MGSA (XP-5147Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGRO/MGSA. Western Blot: To detect hGRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO/MGSA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | GRO/MGSA antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
