Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal breast. The recommended concentration is 0.125 mg/mL-0.0625 mg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
To detect Human Heregulin-beta1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human Heregulin-beta1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human Heregulin-beta1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human Heregulin-beta1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human Heregulin-beta1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Heregulin-beta1(38-253) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Heregulin-beta1.| Product Name | Heregulin-beta1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Human Heregulin-B1. Human Heregulin-B1 specific antibody was purified by affinity chromatography employing an immobilized Human Heregulin-B1 matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hHeregulin-B1 (1.0 ng/mL), a concentration of 0.1-1.1 μg/mL of this antibody is required. ELISA: In a sandwich ELISA To detect hHeregulin-B1 (using 100 uL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction withour biotinylated Anti-Human hHeregulin-B1as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hHeregulin-B1. Western Blot: To detect hHeregulin-B1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHeregulin-B1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | NRG1 |
|---|---|
| Alternative Names | GGF HGL HRG NDF ARIA GGF2 HRG1 HRGA SMDF MST131 GGF Pro-neuregulin-1 membrane-bound isoform Acetylcholine receptor-inducing activity Pro-NRG1 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal breast. The recommended concentration is 0.125 mg/mL-0.0625 mg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
To detect Human Heregulin-beta1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human Heregulin-beta1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human Heregulin-beta1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human Heregulin-beta1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human Heregulin-beta1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Heregulin-beta1(38-253) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Heregulin-beta1.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hHeregulin-B1 (1.0 ng/mL), a concentration of 0.1-1.1 μg/mL of this antibody is required. ELISA: In a sandwich ELISA To detect hHeregulin-B1 (using 100 uL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction withour biotinylated Anti-Human hHeregulin-B1as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hHeregulin-B1. Western Blot: To detect hHeregulin-B1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHeregulin-B1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
