Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Flow cytometric analysis of NCI-H460 cells using NRG1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of anti-NRG1 Antibody in SK-BR-3 cell line lysates (35ug/lane)| Product Name | NRG1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pro-neuregulin-1, membrane-bound isoform, Pro-NRG1, Neuregulin-1, Acetylcholine receptor-inducing activity, ARIA, Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin, HRG, Neu differentiation factor, Sensory and motor neuron-deriv |
| Product description | Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells.1) Stove, C., et al., J. Invest. Dermatol. 121(4):802-812 (2003). |
| Immunogen | This NRG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-229 amino acids from the Central region of human NRG1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | NRG1 |
|---|---|
| Alternative Names | Pro-neuregulin-1 membrane-bound isoform Pro-NRG1 Neuregulin-1 Acetylcholine receptor-inducing activity ARIA Breast cancer cell differentiation factor p45 Glial growth factor Heregulin HRG Neu differentiation factor Sensory and motor neuron-deriv |
| Molecular Weight(MW) | 70 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Flow cytometric analysis of NCI-H460 cells using NRG1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of anti-NRG1 Antibody in SK-BR-3 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
